Радіоелектроніка та телекомунікації
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Вісник Національного університету "Львівська політехніка"
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Item Відображення результатів вимірювання параметрів динамічних мікрооб’єктів телевізійним сканувальним оптичним мікроскопом(Видавництво Львівської політехніки, 2015) Шклярський, В. І.; Матієшин, Ю. М.Розглянуто питання відображення результатів вимірювання параметрів динамічних мікрооб’єктів за допомогою телевізійного сканувального оптичного мікроскопа. При цьому мікрооб’єкти можуть бути як одиничними, так і перебувати у групі з декількох окремих динамічних мікрооб’єктів. Наведено принципи та алгоритм роботи мікроскопа, що забезпечують відображення результатів вимірювання параметрів як окремих динамічних мікрооб’єктів, так і усієї групи. Television scanning optical microscope (TSOM) combined with computer support allows control of its modes in general, individual nodes, image processing and transmission capabilities, storage and playback of the data in the right form for the operator (graphs, tables, diagrams, etc.). An example of the effective use of computer technology symbiosis and measuring systems are complex CASA (Computer-aided sperm analysis). Graphic display of measurement results methods are widely used in cytophotometry to analyze the distribution of absorbing substances in the cells processes. These ways of quantitative assessment of cytological microobjects structure (MO) are based on microphotometry: histogram and topograms, calculating of the surface relief indicator and texture coefficient and so on. It is possible to represent MO in digital or gray-scale fields, as well as pseudorelief. Solving problems related to the display of the velocity vector in the form that is most visible to the operator and can give him the maximum amount of information about the behavior of MO, as well as to simplify and speed up its work, is very important. This article contains analysis ways of display the results of measurement parameters in TSOM as single dynamic MO and dynamic individual MO which are in a group of several MO moving chaotically – with variable velocity and direction of motion. The basic principles of TSOM in determining the dynamic parameters of individual MO and averaged parameters of several MO as a whole. A vector representation of measured values used in vector-cardiography, which originated in the development of electrocardiography. Presenting measure as vektorogram enables display bias in the direction of MO. The pronounced tendency of MO movement within a certain angular sector at a certain velocity rate may indicate different defects of physiological character in the structure of the MO. Examples are the different forms of violation of human sperm structure, which affect the nature of their movement velocity and the consequent loss of the ability to fertilize. MO real movement usually occurs in 3-D space. In this case, three-dimensional model is built as vektorogram. It gives researchers the complete information on the movement of MO, providing at the same time to assess the actual relationships between different components of the movement. Under the group of dynamic MO will realize several separate dynamic MO, which are within sight of TSOM have chaotic (random) motion with variable velocity and direction of movement. Often in modern medicine, microbiology, ecology and many others there is a need to analyze the dynamic parameters not only individual MO, but averaged dynamic parameters of the group as a whole. To do this successfully, you can use all the above listed ways reflect the results of measuring the dynamics ofMO.Item Точність визначення параметрів двох динамічних мікрооб'єктів за допомогою телевізійного сканувального оптичного мікроскопа(Видавництво Львівської політехніки, 2013) Шклярський, В. І.; Матієшин, Ю. М.; Баланюк, Ю. В.; Гудзь, Б. В.At the present stage of technological development there are more problems associated with micro- and nanotechnology. Among such problems occupy an important place task analysis of dynamic processes and several dynamic microobjects (MO) parameters simultaneously in view of the microscope in different fields (medicine, microbiology, ecology, microelectronics etc.). Identification number of dynamic parameters (for example, mobility) of some living cells and their components occurs mainly by using optical microscopy methods. To determine the mobility is most often used in two ways: 1) the method of fluorescence microphotographs analysis; 2) the method of quantitative fluorescence confocal laser scanning microscopy. Using these methods were quantitatively and qualitatively describes the process of intracellular transport, particularly cytoplasm-nuclear, kinetic accumulation in the nucleus of cells of different types of proteins, localization of drugs aimed at developing ways to intracellular delivery of anticancer drugs. To solve these and many such important tasks in the fields of medicine and microbiology can be successfully used television scanning optical microscope (TSOM) based on cathode ray tubes ultrahigh resolution, as provided in this scan resolution up to 0.1 ... 0.2 micron. This article contains analysis of the capabilities of this microscope in the case of the simultaneous study of several MO, which are move randomly - with variable speed and direction of motion. The basic principles of TSOM work in determining the dynamic parameters of separate MO and averaged parameters of several MO are submitted. Track each individual MO occurs by the use of scan mode of miniraster, the center of which the current frame is formed with coordinates that correspond to coordinates of the center of the MO in the previous frame scan. Interval of definition of coordinate position of each MO in sight of TSOM corresponds to the duration of one frame scanning at a constant speed scanning. Dimensions of the scanning raster should be reduced to the values 1...10% of the full-scale raster, while receiving miniraster. However, a scan should be used for MO of proportionate size with minimal scanning spot that move randomly at high speed. Among the MO for which the use of the scanning mode is not appropriate, you can select one or several MO simultaneously with large size (5...40 % of the full-scale raster in the plane of study). For such MO should be used only scanning with a full-scale square raster. Based on these considerations, we construct a mathematical model of the scanning process and analyze the significance of dynamic parameters which can be determined by TSOM using scan mode with a full-scale raster and tracking eachMO by miniraster. Розглядаються питання використання телевізійного сканувального оптичного мікроскопа для визначення різних параметрів динамічних мікрооб’єктів, які перебувають у групі, із застосуванням режимів сканування повноформатним растром та слідкування за кожним окремим мікрооб’єктом за допомогою сканувального мінірастра. Наведені принципи роботи мікроскопа, що забезпечують визначення параметрів як окремих динамічних мікрооб’єктів, так і групи загалом.