Obtaining biomass of Echinacea purpurea in vitro

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dc.citation.epage157
dc.citation.issue1
dc.citation.journalTitleХімія, технологія речовин та їх застосування
dc.citation.spage153
dc.citation.volume8
dc.contributor.affiliationНаціональний університет “Львівська політехніка”
dc.contributor.affiliationУніверситет Лілля
dc.contributor.affiliationLviv Polytechnic National University
dc.contributor.affiliationUniversity of Lille
dc.contributor.authorБлізніченко, А. І.
dc.contributor.authorПетріна, Р. О.
dc.contributor.authorЮськів, С. Л.
dc.contributor.authorДевір, К.
dc.contributor.authorBliznichenko, A. I.
dc.contributor.authorPetrina, R. O.
dc.contributor.authorYuskiv, S. L.
dc.contributor.authorDeweer, C.
dc.coverage.placenameЛьвів
dc.coverage.placenameLviv
dc.date.accessioned2026-03-30T09:52:22Z
dc.date.created2025-02-27
dc.date.issued2025-02-27
dc.description.abstractУ роботі досліджено можливість введення Echinacea purpurea в культуру in vitro з метою отримання калусної біомаси. Описано методику стерилізації насіння та вирощування проростків на середовищі Мурасіге-Скуга. Експериментально визначено вплив різних комбінацій регуляторів росту (ІОК, БАП, НОК, 2,4-Д) на утворення калусу з листкових, стеблових і кореневих експлантів. Встановлено, що вищі ростові характеристики калусної біомаси на середовищі з додаванням регуляторів росту ІОК та БАП у співвідношенні 2:1 мг/л, ніж НОК, 2,4-Д у співвідношенні 1:1 мг/л. На листкових і стеблових експлантах утворювався калус жовто-зеленого і світло-коричневого кольору. Кореневі експланти калюсогенезу не проявляли.
dc.description.abstractThis work investigated the possibility of introducing Echinacea purpurea into in vitro culture in order to obtain callus biomass. The method of seed sterilization and seedling cultivation on Murashige-Skoog medium was described. The effect of various combinations of growth regulators (IAA, BAP, NAA, 2,4-D) on callus formation from leaf, stem and root explants was experimentally determined. It was found that the formation of callus biomass occurs better on a medium with the addition of growth regulators IAA and BAP in a ratio of 2:1 mg/l than NAA, 2,4-D in a ratio of 1:1 mg/l. Callus of yellow-green and light brown color was formed on leaf and stem explants. Root explants did not show callusogenesis.
dc.format.extent153-157
dc.format.pages5
dc.identifier.citationObtaining biomass of Echinacea purpurea in vitro / A. I. Bliznichenko, R. O. Petrina, S. L. Yuskiv, C. Deweer // Chemistry, Technology and Application of Substances. — Lviv : Lviv Politechnic Publishing House, 2025. — Vol 8. — No 1. — P. 153–157.
dc.identifier.citationenObtaining biomass of Echinacea purpurea in vitro / A. I. Bliznichenko, R. O. Petrina, S. L. Yuskiv, C. Deweer // Chemistry, Technology and Application of Substances. — Lviv : Lviv Politechnic Publishing House, 2025. — Vol 8. — No 1. — P. 153–157.
dc.identifier.doidoi.org/10.23939/ctas2025.01.153
dc.identifier.urihttps://ena.lpnu.ua/handle/ntb/124857
dc.language.isoen
dc.publisherВидавництво Львівської політехніки
dc.publisherLviv Politechnic Publishing House
dc.relation.ispartofХімія, технологія речовин та їх застосування, 1 (8), 2025
dc.relation.ispartofChemistry, Technology and Application of Substances, 1 (8), 2025
dc.relation.references1. Mamedova, V. N. (2023). Advantages of Growing Plants by in-Vitro Method. International Journal of Scientific Trends, 2(4), 19–23. Retrieved from https://scientifictrends.org/index.php/ijst/article/view/86
dc.relation.references2. Kulak, V.; Longboat, S.; Brunet, N.D.; Shukla, M.; Saxena, P. (2022) In Vitro Technology in Plant Conservation: Relevance to Biocultural Diversity. Plants, 11, 503. https://doi.org/10.3390/plants11040503
dc.relation.references3. Anjali, Kopal Singh, Dr. Gurshaminder Singh (2024). Fundamental of Tissue Culture and it’s Future Prospects in Crop Improvement. International Journal of Scientific Research & Engineering Trends, 10 (5), ISSN (Online): 2395-566X https://doi.org/10.61137/ijsret.vol.10.issue5.270
dc.relation.references4. Burlou-Nagy C., Banica F., Jurca T., Vicas L.G., & Marian E., et al. (2022) Echinacea purpurea (L.) Moench: Biological and Pharmacological Properties - A Review. Plants., 11, 1244. https://doi.10.3390/plants11091244
dc.relation.references5. Ciganovic P., Jakupovic L., Momchev P., Nižic Nodilo L., Hafner A. & Zovko Koncic M. (2023) Extraction Optimization, Antioxidant, Cosmeceutical and Wound Healing Potential of Echinacea purpurea. Glycerolic Extracts. Molecules, 28, 1177. https://doi.10.3390/molecules28031177
dc.relation.references6. El-Ashmawy, N., El-Zamarany, E., Salem, M., El-Bahrawy, H., & Al-Ashmawy, G. (2015). In vitro and in vivo studies of the immunomodulatory effect of Echinacea purpurea on dendritic cells. Journal of Genetic Engineering and Biotechnology, 13(2), 185–192. https://doi.10.1016/j.jgeb.2015.05.002
dc.relation.references7. Li, T. S. C. (1998). Echinacea: Cultivation and Medicinal Value. HortTechnology, 8(2), 122–129. https://doi.org/10.21273/horttech.8.2.122
dc.relation.references8. Nilanthi, D., Chen, X.-L., Zhao, F.-C., Yang, Y.-S., & Wu, H. (2009). Induction of Tetraploids from Petiole Explants through Colchicine Treatments inEchinacea purpureaL. Journal of Biomedicine and Biotechnology, 2009, 1–7. https://doi.org/10.1155/2009/343485
dc.relation.references9. Pospelov, S., Zdor, V., Mishchenko, O., Pospelova, A., & Kovalenko, N. (2020). Model of creation of productive agrocenosis of Echinacea. E3S Web of Conferences, 222, 02048. https://doi.org/10.1051/e3sconf/202022202048
dc.relation.references10. Makukha, O. (2021). Technological Improvement of Echinacea purpurea Cultivation. Ecological Engineering & Environmental Technology, 22(5), 89–96. https://doi.org/10.12912/27197050/139336
dc.relation.references11. Coker P.S., & Camper N.D. (2000) In vitro culture of Echinacea purpurea L. J. of Herbs Spices an Medicinal Plants, 7(4), 1-7. https://doi.10.1300/J044v07n04_01
dc.relation.references12. Kristen L. Choffe, Jerrin M. R. Victor, Susan J. Murch & Praveen K. Saxena. (2000) In Vitro Regeneration of Echinacea purpurea L.: Direct Somatic Embryogenesis and Indirect Shoot Organogenesis in Petiole Culture / In vitro Cellular & Developmental Biology. Plant, 36, 1, 30-36. https://doi.10.1007/s11627-000-0008-4
dc.relation.references13. Dahanayake, N., Chen, X.-L., Zhao, F.-C., & Yang, Y.-S. (2011). An Efficient In Vitro Propagation Culture Protocol for Purple Coneflower (Echinacea purpurea L.). Tropical Agricultural Research and Extension, 13(2)29–32. https://doi.10.4038/tare.v13i2.3135
dc.relation.references14. Maggini, V., Mengoni, A., Gallo, E. R. G., Biffi, S., Fani, R., Firenzuoli, F., & Bogani, P. (2019). Tissue specificity and differential effects on in vitro plant growth of single bacterial endophytes isolated from the roots, leaves and rhizospheric soil of Echinacea purpurea. BMC Plant Biology, 284(2019), 30-36. https://doi.10.1186/s12870-019-1890-z
dc.relation.referencesen1. Mamedova, V. N. (2023). Advantages of Growing Plants by in-Vitro Method. International Journal of Scientific Trends, 2(4), 19–23. Retrieved from https://scientifictrends.org/index.php/ijst/article/view/86
dc.relation.referencesen2. Kulak, V.; Longboat, S.; Brunet, N.D.; Shukla, M.; Saxena, P. (2022) In Vitro Technology in Plant Conservation: Relevance to Biocultural Diversity. Plants, 11, 503. https://doi.org/10.3390/plants11040503
dc.relation.referencesen3. Anjali, Kopal Singh, Dr. Gurshaminder Singh (2024). Fundamental of Tissue Culture and it’s Future Prospects in Crop Improvement. International Journal of Scientific Research & Engineering Trends, 10 (5), ISSN (Online): 2395-566X https://doi.org/10.61137/ijsret.vol.10.issue5.270
dc.relation.referencesen4. Burlou-Nagy C., Banica F., Jurca T., Vicas L.G., & Marian E., et al. (2022) Echinacea purpurea (L.) Moench: Biological and Pharmacological Properties - A Review. Plants., 11, 1244. https://doi.10.3390/plants11091244
dc.relation.referencesen5. Ciganovic P., Jakupovic L., Momchev P., Nižic Nodilo L., Hafner A. & Zovko Koncic M. (2023) Extraction Optimization, Antioxidant, Cosmeceutical and Wound Healing Potential of Echinacea purpurea. Glycerolic Extracts. Molecules, 28, 1177. https://doi.10.3390/molecules28031177
dc.relation.referencesen6. El-Ashmawy, N., El-Zamarany, E., Salem, M., El-Bahrawy, H., & Al-Ashmawy, G. (2015). In vitro and in vivo studies of the immunomodulatory effect of Echinacea purpurea on dendritic cells. Journal of Genetic Engineering and Biotechnology, 13(2), 185–192. https://doi.10.1016/j.jgeb.2015.05.002
dc.relation.referencesen7. Li, T. S. C. (1998). Echinacea: Cultivation and Medicinal Value. HortTechnology, 8(2), 122–129. https://doi.org/10.21273/horttech.8.2.122
dc.relation.referencesen8. Nilanthi, D., Chen, X.-L., Zhao, F.-C., Yang, Y.-S., & Wu, H. (2009). Induction of Tetraploids from Petiole Explants through Colchicine Treatments inEchinacea purpureaL. Journal of Biomedicine and Biotechnology, 2009, 1–7. https://doi.org/10.1155/2009/343485
dc.relation.referencesen9. Pospelov, S., Zdor, V., Mishchenko, O., Pospelova, A., & Kovalenko, N. (2020). Model of creation of productive agrocenosis of Echinacea. E3S Web of Conferences, 222, 02048. https://doi.org/10.1051/e3sconf/202022202048
dc.relation.referencesen10. Makukha, O. (2021). Technological Improvement of Echinacea purpurea Cultivation. Ecological Engineering & Environmental Technology, 22(5), 89–96. https://doi.org/10.12912/27197050/139336
dc.relation.referencesen11. Coker P.S., & Camper N.D. (2000) In vitro culture of Echinacea purpurea L. J. of Herbs Spices an Medicinal Plants, 7(4), 1-7. https://doi.10.1300/J044v07n04_01
dc.relation.referencesen12. Kristen L. Choffe, Jerrin M. R. Victor, Susan J. Murch & Praveen K. Saxena. (2000) In Vitro Regeneration of Echinacea purpurea L., Direct Somatic Embryogenesis and Indirect Shoot Organogenesis in Petiole Culture, In vitro Cellular & Developmental Biology. Plant, 36, 1, 30-36. https://doi.10.1007/s11627-000-0008-4
dc.relation.referencesen13. Dahanayake, N., Chen, X.-L., Zhao, F.-C., & Yang, Y.-S. (2011). An Efficient In Vitro Propagation Culture Protocol for Purple Coneflower (Echinacea purpurea L.). Tropical Agricultural Research and Extension, 13(2)29–32. https://doi.10.4038/tare.v13i2.3135
dc.relation.referencesen14. Maggini, V., Mengoni, A., Gallo, E. R. G., Biffi, S., Fani, R., Firenzuoli, F., & Bogani, P. (2019). Tissue specificity and differential effects on in vitro plant growth of single bacterial endophytes isolated from the roots, leaves and rhizospheric soil of Echinacea purpurea. BMC Plant Biology, 284(2019), 30-36. https://doi.10.1186/s12870-019-1890-z
dc.relation.urihttps://scientifictrends.org/index.php/ijst/article/view/86
dc.relation.urihttps://doi.org/10.3390/plants11040503
dc.relation.urihttps://doi.org/10.61137/ijsret.vol.10.issue5.270
dc.relation.urihttps://doi.10.3390/plants11091244
dc.relation.urihttps://doi.10.3390/molecules28031177
dc.relation.urihttps://doi.10.1016/j.jgeb.2015.05.002
dc.relation.urihttps://doi.org/10.21273/horttech.8.2.122
dc.relation.urihttps://doi.org/10.1155/2009/343485
dc.relation.urihttps://doi.org/10.1051/e3sconf/202022202048
dc.relation.urihttps://doi.org/10.12912/27197050/139336
dc.relation.urihttps://doi.10.1300/J044v07n04_01
dc.relation.urihttps://doi.10.1007/s11627-000-0008-4
dc.relation.urihttps://doi.10.4038/tare.v13i2.3135
dc.relation.urihttps://doi.10.1186/s12870-019-1890-z
dc.rights.holder© Національний університет “Львівська політехніка”, 2025
dc.subjectрослинна сировина
dc.subjectкультивування
dc.subjectкалусна біомаса
dc.subjectEchinacea purpurea
dc.subjectplant raw materials
dc.subjectcultivation
dc.subjectcallus biomass
dc.subjectEchinacea purpurea
dc.titleObtaining biomass of Echinacea purpurea in vitro
dc.title.alternativeОтримання біомаси Echinacea purpurea in vitro
dc.typeArticle

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Chemistry, Technology and Application of Substances. – 2025. – Vol. 8, No. 1