Hydrotrope-induced micellar phase of sodium dodecyl sulfate as new detergent for extraction and stabilization of proteins

Simple item page
dc.citation.epage201
dc.citation.issue2
dc.citation.journalTitleChemistry & Chemical Technology
dc.citation.spage196
dc.citation.volume12
dc.contributor.affiliationTaras Shevchenko National University of Kyiv
dc.contributor.authorStarova, Viktoriia
dc.contributor.authorKulichenko, Sergey
dc.coverage.placenameLviv
dc.date.accessioned2019-06-18T13:20:38Z
dc.date.available2019-06-18T13:20:38Z
dc.date.created2018-01-20
dc.date.issued2018-01-20
dc.description.abstractДані рентгенофазового аналізу та інфра- червоної спектроскопії підтверджують гелеподібну природу міцелярної фази додецилсульфату натрію індукованої добав- ками хлориду натрію та саліцилової кислоти. Така міцелярна фаза переважно складається з води (ωH2O > 50 %), яка формує внутрішньомолекулярні водневі зв’язки з основними компонен- тами міцелярно-екстракційної системи. Встановлена здат- ність даної міцелярної фази кількісно вилучати білки (R > 94 %) із збереженням їх каталітичної активності та дисперсності.
dc.description.abstractX-ray phase analysis and IR spectroscopy data have confirmed a gel-like nature of sodium dodecyl sulfate micellar phase induced by sodium chloride and salicylic acid additives. This phase essentially consists of water (ωH2O > 50 %), which is connected with main components of the micellar-extraction system through intermolecular hydrogen bonds. Capacity of such micellar phase to quantitatively extract of proteins (R > 94 %) with saving of their catalytic activities and dispersity was found.
dc.format.extent196-201
dc.format.pages6
dc.identifier.citationStarova V. Hydrotrope-induced micellar phase of sodium dodecyl sulfate as new detergent for extraction and stabilization of proteins / Viktoriia Starova, Sergey Kulichenko // Chemistry & Chemical Technology. — Lviv : Lviv Politechnic Publishing House, 2018. — Vol 12. — No 2. — P. 196–201.
dc.identifier.citationenStarova V. Hydrotrope-induced micellar phase of sodium dodecyl sulfate as new detergent for extraction and stabilization of proteins / Viktoriia Starova, Sergey Kulichenko // Chemistry & Chemical Technology. — Lviv : Lviv Politechnic Publishing House, 2018. — Vol 12. — No 2. — P. 196–201.
dc.identifier.urihttps://ena.lpnu.ua/handle/ntb/45141
dc.language.isoen
dc.publisherLviv Politechnic Publishing House
dc.relation.ispartofChemistry & Chemical Technology, 2 (12), 2018
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dc.relation.referencesen[1] Sova V., KusaikinM.:Methodicheskoe Rukovodstvo dlia Practicheskykh Zaniatiy. Izd-vo Dalnevost. Univ., Vladivostok2006.
dc.relation.referencesen[2] Allard J., Baird A., Davidson G., et al., Sci. Justice, 2007, 47,160. https://doi.org/10.1016/j.scijus.2007.09.010
dc.relation.referencesen[3] JohnsonM., Yakimchuk K.:Mater. Methods, 2013, 3, 163.https://doi.org/10.13070/mm.ru.3.163
dc.relation.referencesen[4]Matar-Merheb R., Rhimi M., Leydier A. et al., Plos One, 2011,6. https://doi.org/10.1371/journal.pone.0018036
dc.relation.referencesen[5] Kulichenko S., Starova V., Chem. Pap., 2010, 64, 98.https://doi.org/10.2478/s11696-009-0086-7
dc.relation.referencesen[6] González de la Vara L., Alfaro B., Anal. Biochem., 2009, 387,280. https://doi.org/10.1016/j.ab.2009.01.035
dc.relation.referencesen[7] Starova V., Kulichenko S., J. Anal. Chem., 2010, 65, 1244.https://doi.org/10.1134/S1061934810120038
dc.relation.referencesen[8] Knorre D., Myzina S., Biochemistry: AManual for Universities. Nova Science Books and Jrnls, New York 1998.
dc.relation.referencesen[9] Kulichenko S., Visnyk Kyiv. Nats. Univ., 1997, 34, 55.
dc.relation.referencesen[10] Zhaporozhets O., Nadzhafova O., Smyk N. et al., Rukovodstvo dlia Laboratornykh Rabot po Analiticheskoi Khimii. Izd-vo Kyiv. Univ., Kyiv 2005.
dc.relation.referencesen[11] Severin S., Soloveva G. (Ed.): Seminar po Biokhimii. Uchebnoe Posobie. Izd-vo Moskov. Gos. Univ., Moskva 1989.
dc.relation.referencesen[12] Klenin V., Shepolev S., Lavrushin V., Characteristicheskie Funktsii Rasseivania Sveta Dispersnykh System. Izd-vo Saratov. Univ., Saratov 1977.
dc.relation.referencesen[13] Nadzhafova O., DrozdovaM., Bosak V., Visnyk Kyiv. Nats. Univ., 2006, 43, 23.
dc.relation.referencesen[14] Simon Bates. Advanced Analysis of Non-Crystalline (X-ray Amorphous) Materials and Dispersions. www. Tricliniclabs.com.
dc.relation.referencesen[15] Davletshin A., Silvesrtova I., Zubov V. et al., VestnikMoscov. Univ., 1998, 39, 272.
dc.relation.urihttps://doi.org/10.1016/j.scijus.2007.09.010
dc.relation.urihttps://doi.org/10.13070/mm.ru.3.163
dc.relation.urihttps://doi.org/10.1371/journal.pone.0018036
dc.relation.urihttps://doi.org/10.2478/s11696-009-0086-7
dc.relation.urihttps://doi.org/10.1016/j.ab.2009.01.035
dc.relation.urihttps://doi.org/10.1134/S1061934810120038
dc.rights.holder© Національний університет „Львівська політехніка“, 2018
dc.rights.holder©Starova V., Kulichenko S., 2018
dc.subjectміцелярна екстракція
dc.subjectдодецилсульфат натрію
dc.subjectбілок
dc.subjectнативний стан
dc.subjectкаталітична активність
dc.subjectmicellar extraction
dc.subjectsodium dodecyl sulfate
dc.subjectprotein
dc.subjectnative state
dc.subjectcatalytic activity
dc.titleHydrotrope-induced micellar phase of sodium dodecyl sulfate as new detergent for extraction and stabilization of proteins
dc.title.alternativeГідротроп-індукована міцелярна фаза додецилсульфату натрію як новий детергент для вилучення та стабілізації білків
dc.typeArticle

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Chemistry & Chemical Technology. – 2018. – Vol. 12, No. 2