Quantitative Determination of Ephedrine Hydrochloride in Pharmaceutical Injections by Highly Sensitive Turbidimetric and Reversed-Phase Combined with UFLC Methods

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dc.citation.epage274
dc.citation.issue2
dc.citation.spage269
dc.contributor.affiliationUniversities of Baghdad
dc.contributor.authorJeber, Jalal N.
dc.coverage.placenameЛьвів
dc.coverage.placenameLviv
dc.date.accessioned2020-03-02T12:28:11Z
dc.date.available2020-03-02T12:28:11Z
dc.date.created2019-02-28
dc.date.issued2019-02-28
dc.description.abstractЗа допомогою турбидиметричного методу та обернено-фазної надшвидкої рідинної хроматографії (НРХ) визначено кількість гідрохлориду ефедрину у фармацевтичних ін‘єкціях. Першим методом на основі турбидиметричних величин утвореного жовтувато-білого осаду у вигляді суспензії визначено концентрацію гідрохлориду ефедрину. Суспензія утво- рюється внаслідок реакції між гідрохлоридом ефедрину та флуоромолібденовою кислотою, яку використовували як реа- гент. Досліджено її фізико-хімічні характеристики та побудо- вано калібрувальні графіки ефедрину. У другому способі (НРХ) використано рухому фазу метанол-вода (55:45, об. %; рН води 3,5). Кількість гідрохлориду ефедрину визначали з вико- ристанням УФ-детектора при 260 нм. Лінійність ефедрину встановлено в діапазоні 0,09–0,39 ммоль·л-1, а межа чутливості для турбидиметричного та НРХ методу становила 0,4 та 0,0044 ммоль·л-1, відповідно. Показано, що розроблені методи можна успішно застосовувати для кількісного визначення гідро- хлориду ефедрину в лабораторних препаратах (стандарт) та ко- мерційних фармацевтичних ін‘єкціях. Відносні стандартні від- хилення обох методів знаходяться в інтервалі 0,65–1,69%, що вказує на коректну відтворюваність і високу точність методів.
dc.description.abstractIn this paper, turbidimetric and reversed-phase ultra-fast liquid chromatography (UFLC) methods were described for the quantitative determination of ephedrine hydrochloride in pharmaceutical injections form. The first method is based on measuring the turbidimetric values for the formed yellowish white precipitate in suspension status in order to determine the ephedrine hydrochloride concentration. The suspended substance is formed as a result of the reaction of ephedrine hydrochloride with phosphomolybdic acid which was used as a reagent. The physical and chemical characteristics of the complex were investigated. The calibration graphs of ephedrine were established by turbidity method. While the second method (UFLC) was conducted using the methanol-water (55+45, v/v) as the mobile phase with adjusted water pH 3.5. The ephedrine hydrochloride was detected and measured using UV detector at 260 nm. The linearity of ephedrine was obtained in the range of 0.09–0.39 mmol·l-1. The detection limits (LOD) for the ephedrine hydrochloride were found to be 0.4 and 0.0044 mmol·l-1 by turbidity and UFLC, respectively. The developed methods were successfully applied for the quantitative determination of ephedrine hydrochloride in laboratory preparations (standard) and in commercial pharmaceutical injections. The two methods have given relative standard deviations (R.S.D.) in the range of 0.65–1.69 %, which indicates reasonable repeatability and high precision of both methods.
dc.format.extent269-274
dc.format.pages6
dc.identifier.citationJeber J. N. Quantitative Determination of Ephedrine Hydrochloride in Pharmaceutical Injections by Highly Sensitive Turbidimetric and Reversed-Phase Combined with UFLC Methods / Jalal N. Jeber // Chemistry & Chemical Technology. — Lviv : Lviv Politechnic Publishing House, 2019. — Vol 13. — No 2. — P. 269–274.
dc.identifier.citationenJeber J. N. Quantitative Determination of Ephedrine Hydrochloride in Pharmaceutical Injections by Highly Sensitive Turbidimetric and Reversed-Phase Combined with UFLC Methods / Jalal N. Jeber // Chemistry & Chemical Technology. — Lviv : Lviv Politechnic Publishing House, 2019. — Vol 13. — No 2. — P. 269–274.
dc.identifier.urihttps://ena.lpnu.ua/handle/ntb/46468
dc.language.isoen
dc.publisherВидавництво Львівської політехніки
dc.publisherLviv Politechnic Publishing House
dc.relation.ispartofChemistry & Chemical Technology, 2 (13), 2019
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dc.relation.urihttps://doi.org/10.1016/0021-9673(92)85575-E
dc.relation.urihttps://doi.org/10.1007/s11655-014-1952-x
dc.relation.urihttps://doi.org/10.1007/978-3-319-32328-2_11
dc.relation.urihttps://doi.org/10.1002/ptr.1337
dc.relation.urihttps://doi.org/10.1016/S0731-7085(02)00065-1
dc.relation.urihttps://doi.org/10.1016/j.saa.2013.03.038
dc.relation.urihttps://doi.org/10.5740/jaoacint.17-0078
dc.relation.urihttps://doi.org/10.1007/BF00322242
dc.relation.urihttps://doi.org/10.1002/dta.1963
dc.relation.urihttps://doi.org/10.1016/S1570-0232(03)00222-8
dc.relation.urihttps://doi.org/10.1080/10826076.2017.1287723
dc.relation.urihttps://doi.org/10.1007/s10337-016-3170-5
dc.relation.urihttps://doi.org/10.1016/S0731-7085(99)00103-X
dc.relation.urihttps://doi.org/10.1248/yakushi1947.120.6_583
dc.relation.urihttps://doi.org/10.1016/S0379-0738(03)00101-4
dc.relation.urihttps://doi.org/10.1016/S1570-0232(02)00522-6
dc.relation.urihttps://doi.org/10.1093/chromsci/bmw082
dc.relation.urihttps://doi.org/10.1016/j.chroma.2011.09.078
dc.relation.urihttps://doi.org/10.1016/j.jchromb.2017.10.062
dc.relation.urihttps://doi.org/10.1016/j.forsciint.2017.06.026
dc.relation.urihttps://doi.org/10.1016/j.snb.2016.05.058
dc.relation.urihttps://doi.org/10.1016/j.talanta.2012.10.067
dc.relation.urihttps://doi.org/10.1016/j.jchromb.2015.12.027
dc.relation.urihttps://doi.org/10.1080/10826076.2017.1300171
dc.relation.urihttps://doi.org/10.1016/j.jchromb.2016.11.039
dc.relation.urihttps://doi.org/10.1093/chromsci/bmw165
dc.relation.urihttps://doi.org/10.1002/dta.1803
dc.rights.holder© Національний університет „Львівська політехніка“, 2019
dc.rights.holder© Jeber J., 2019
dc.subjectобернено-фазна надшвидка рідинна хроматографія
dc.subjectгідрохлорид ефедрину
dc.subjectтурбидиметрія
dc.subjectфармацевтичні препарати
dc.subjectUFLC
dc.subjectephedrine hydrochloride
dc.subjectturbidity
dc.subjectpharmaceutical preparations
dc.titleQuantitative Determination of Ephedrine Hydrochloride in Pharmaceutical Injections by Highly Sensitive Turbidimetric and Reversed-Phase Combined with UFLC Methods
dc.title.alternativeКількісне визначення гідрохлориду ефедріну у фармацевтичних ін‘екціях високочутливим турбидиметричним методом і обернено-фазною надшвидкою рідинною хроматографією
dc.typeArticle

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Chemistry & Chemical Technology. – 2019. – Vol. 13, No. 2